We have documented various levels of fluoride in groundwater, running water (i.e streams, canals, river) and lake water in 130 samples collected from various sources in Mysore District. Mysore is one of the most popular tourist places in India. Fluoride is one of the parameter of water analysis, which is non-degradable and persists in the environment. Fluoride was assessed by Zirconyl- SPADNS method. Fluoride level varied from 0.2 mg/L to 3.0 mg/L with the highest level at Dalvoy Lake (3.0 ppm) followed by Lingambudi Borewell water (2.9 ppm) giving a cautious alarm for an awareness to the Mysoreans.Water samples from north-eastern part of Piriyapatna, a small pocket of southern part of H.D.Kote and an extreme southern part of Mysore taluk (urban) were having fluoride concentration above acceptable range of WHO and BIS standard.