Bioaccumulation of heavy metals (Cu, Cd, Ni, Fe and Pb) was examined by atomic absorption spectrophotometer in sediment, water and fish samples of two different lakes; freshwater and sewage fed water namely Upper and Shahpura Lake respectively of Bhopal, (M.P.) India. All these trace metals were greater in polluted lake as compared to freshwater lake, except Pb. The experimental results clearly indicated heavy metal accumulation in different trophic level of both lakes.