Camellia sinensis originated in South East Asia, specifically around the intersection in the point of confluence of the lands of northeast India, north Burma, southwest China and Tibet. The plant was introduced to more than 52 countries, from this ‘centre of origin’." Instant coffee is a beverage prepared by various processes in which again it is dehydrated into the form of powder or granules. These can be rehydrated with hot water to provide a drink similar (though not identical) to conventional coffee. Chicory, used as a coffee substitute and additive with other adulterants like sugar beet and mustard seeds used as an ingredient of the mixed coffee, introduced during the coffee crisis happened in 1976-79. According to traditional folklore, long-term use of chicory as a coffee substitute may damage human retinal tissue, with dimming of vision over time and other long term effects. Similarly, copper salts are being used commonly as an eradicant and protectant against a fungus. Therefore there is an urgent need of detecting the copper bioconcentration in the samples, hence present problem has been sorted out and it was found that no absorption or the uptake of the salt by the plant and ultimate crop or its allied product is safe for human consumption.