Physico-chemical analysis of Well Water of Kalmeshwar Tehsil, District Nagpur has been studied, All the samples were analysed to assess the water quality parameters like Temperature, Physical appearance, Odor, Turbidity, pH, Chloride, Nitrates, Total hardness, Permanent hardness, Total Dissolved Solids, Sodium, Potassium, Sulphate, Iron, Fluoride, Total alkanity, Dissolved Oxygen, B.O.D, C.O.D. It was found that most of the parameters are within the permissible limit as described by W.H.O. (1978) except Total Hardness and Total Dissolved solids found to be 510 to 1640 mg/L, 930 to 2800 mg/L. respectively.