Isolation of soil mycoflora from Gangtok was observed by serial dilution method, In investigation period 146 colonies of 22 fungal species were observed the maximum percentage contribution of Aspergillus fumigatus, A. niger (12.32%) ,was followed by A. flavus , A. luchensis, Mucor sp. (6.84%) and minimum percentage contribution of Cladosporium sphaerospermum (0.68%). The maximum fungal species belongs to the Ascomycotina 92 colonies, followed by Deuteromycotina 29 colonies, Zygomycotina 18 colonies and mycelia sterilia (white) 7 colonies were observed.