The study of the contamination level of drinking water used and effect of fertilizer industry of aquatic biota in Raghogarh was conducted at 6 stations in Chopan River and 4 stations of surrounding tube wells in year (2004). In the water samples, temperature, pH, conductivity, TDS, hardness, calcium, Magnesium, DO chloride, free ammonia-N were evaluated through analysis. Ii is found that the values of total dissolved solids and free ammonia-N are higher. Total dissolved solids in excessive causes laxative effect, whereas increase in ammonia cause decrease in pH ultimately it is harmful to life, irrigation and drinking purpose.