This paper is aimed at the assesment of nitrate in ground water by various pollution sources such as fertilizer, pesticide and insceticide. 16 water samples were obtained from tube well and hand pumps situtaed at cultivated farms and near villages from Khajuwala (located at canal project area in Bikaner distric of Rajastan state). Water samples were collected in July, Oct (2005) and Jan (2006) and were analyzed for nitrate. Some samples were found to contain nitrate above the permissible limit prescribed by WHO, IS and other organizations. Nitrate concentration in samples of well water situated in cultivated area is found higher than in village area. It was also found to be more in handpump samples than in tube well water sample. This result indicates that nitrate concentration has positive correlation with the use of fertilizers & negative correlation with depth of water table. Nitrate concentration was found to enhance after the monsoon period in the month of Oct (2005), which proves the phenomenon of agricultural leaching of nitrate.