This study is aimed to collect information about the macrofauna diversity of Tawa River. The Tawa River joins to Narmada at district Hoshangabad (Madhya Pradesh). During the study 8 sites were selected for the investigation . collections of samples were done from the eight sampling sites of the river. Collected samples were identified up to species level and their diversity and richness were analyzed. Shannon index for diversity and margalef index for richness were used for the study.Total 48 taxa of macrofauna have been recorded from the identified sampling stations. Benthic fauna of phylum Arthropoda was found in dominant position in the study and phylum Mollusca was found in second position.