District Science Centre campus is not only a green lung for Tirunelveli City, but also aimed to create awareness about the biodiversity among the public. District Science Centre is committed towards developing a green campus, and established a Garden in achieving the commitment. It is the centre for higher learning, being both recreational and educative role; it will continually host a human population consisting of students and common public. The present study focused on tree diversity and their abundance in District Science Centre, Tirunelveli, Tamil Nadu, India. An extensive floristic survey was conducted in 2019. The results of tree diversity in District Science Centre campus showed 80 tree species. Among 80 species confined in 34 families and 68 genera, Polyalthia longofolia was the most dominant and frequent species in the study area. A total of 75 tree species represented by dicot and belong to 63 genera and 32 families were recorded. Out of the identified species, 5 tree species are monocot and belongs to 5 genera and 2 families. In the present study 14 families reported with only one species, 13 families represented by more than 2 species. During the study several anthropogenic activities exploited the tree population in the campus. Still Campus area is was still quite barren, so the entire campus should be lush greenery by new initiative on garden development.