Present  study is directed towards the analysis of  the water quality of the Macferson Lake, Allahabad which is heavily polluted by human activity. The required water samples collected rendomly from different locations of the study area   and analyzed in the department of Environmental Sciences and NRM, SHUATS, Allahabad. The Temperature, EC, pH, TDS, Turbidity, Total hardness, Mg Hardness, Ca Hardness, BOD, DO, Alkalinity, Chloride, and Total coliform of the water samples have been analyzed. To assess the quality of the water each parameter was compared with the standard prescribed by Central pollution control board (CPCB, 2012). It is found that the Water hyacinth and Typha latifolia aquatic plant species were showing higher dominancy over the  Macferson Lake. Both are covering the maximum surface water area of the lake. It is also found that receives species like Lemna minor, Sagitaria latifolia and Hydro-cotyle ranunculoids are found in a very less quantity and restricted to limited areas of the Lake. The findings of the present study help in multi-dimensional aspects and uses water of aforesaid lake including domestic purpose.