This article aims to explain the process of bioremediation of waste oils and found the rate of reaction kinetics model microbes. Samples were taken from liquid waste petroleum oil and gas companies Prabumulih South Sumatra, Indonesia. The isolation process using a medium Zobell’s and produces ten types of bacteria from the group Citrobacter sp, Micrococcaceaekarbon, and Flavobacterium sp. These bacteria’s is used in wastewater treatment bioreactor which have characteristic as follow: operate semi-continuously, using aeration column that contain polyurethane foam, variation of air discharge is 2 and 6 ml/sec, volume waste variation is 5 and 10 liters, 10 liters’ isolate, KNO₃ (640 gram) and K₂HPO₄ (10 gram) as nutrition. The results showed that when bioremediation process contains 10 liters’ liquid waste, 10 liters’ isolate, and 6 ml/sec air discharge, it can reduce pH, specific gravity, BOD level, and the final weight of waste. Remediation process also increases the number of microbes, fatty oils and DO. Microbial maximum productivity is 59.50 with saturated concentration (Ks) at 3.25, and its slope is 0.43. Kinetics equation model following could be formulated as μ = 0.43/s/3.25+s.