Odonates are valuable as indicators of aquatic and terrestrial ecosystem health and also play a vital role as prey and predator to maintain the balance of trophic levels of food chain.  Diversity and distribution of different species of Odonata and physico-chemical properties of water of their habitat in the rural (RA), urban (UA) and tea garden (TG) area of Cachar district of Assam was investigated. A total of fourteen (14) species (larva and adult) were recorded from the three areas. Six species were recorded in RA, four species in TG and six species in UA. Two species Ischnura aurora aurora and Agriocnemis pygmaea pygmaea were recorded common in TG and UA. In TG presence of only two tolerant families indicated that the water quality of the area is polluted. In RA, presence of Aeshnidae indicated relatively better condition of water quality.