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Introduction

石油样品的使用药物due to poverty or ignorance is becoming a source of worry. Unfortunately, the elderly and children are mostly victims. Crude petroleum is usually given to some children when they convulse as anticonvulsant in rural areas of the country. This act is carried out in spite of the mounting evidence that petroleum samples are harmful (Ayalogu, et al.,2001,Igboh,et al.,2001 and Dede,et al.,2002).

Incidentally, the liver is the site where most drugs and other ingested substances are metabolized and detoxified. Assessing the excretory and synthetic functions of the liver can provide valuable clinical information on the state of the liver and could therefore indicate the impact of such substances on the liver (Ayalogu,et al.,2001). The levels of liver enzymes in serum reflect the severity of damage to the hepatocytes by ingested substances. Thus, offer useful information in categorizing damage on the basis of liver cell necrosis (Ayaloguet al.,2001).

Putting these into consideration, we measured the activities of the serum enzymes, specifically the transaminases: ALT and AST. Equally monitored were alkaline phosphatase (ALP) and bilirubin level, noting that these enzymes will indicate any possible damage to the hepatocytes after exposure to crude petroleum (Bonny light), since these enzymes are located in the liver and bile ducts.

Material and Methods

Animals

Thirty six male albino rats with an average body weight of 0.2kg obtained from the Departments of Biochemistry and Pharmacology University of Port Harcourt Rivers State, Nigeria, were used for the study. The animals were acclimatized in the Pharmacology Laboratory for two weeks.

原油(漂亮的光)用于螺栓y was obtained from the Port Harcourt Refinery Company (PHRC), Alesa Eleme. Port Harcourt, Rivers State, Nigeria. These were stored in four litre industrial bottles, well corked, and kept in the dark to avoid lost of any volatile components and reaction with light.

The rats were randomly divide into four groups and were intraperitoneally administered 6.0, 12.0 and 30.0 gkg^-1of crude petroleum (Bonny light) respectively, for first and second month. The doses used were based on LD₅₀determined by Igboh,etal.(2001) which indicated LD₅₀for crude petroleum(Bonny light) as 29.9gkg^-1. At the end of 30 days, three rats were withdrawn from each group for analysis. The control rats were injected normal saline. The animals were fedad libitumwith normal feed and given water freely.

Table 1: Effect of crude petroleum (Bonny light) on serum liver enzyme activities and total bilirubin levels in albino rats Click here to view table

Sample Collection/Analysis

ALP, ALT, AST and total bilurubin were determined from cardiac blood collected with sample bottles without anticoagulant. About 5ml blood was collected for the analysis. The activities of AST and ALT were determined using Reitmen and Frankel (1957) method. ALP activity was determined by the Bower and McComb (1970) method. Total bilirubin was estimated using Mac Donald (1965) method. The statistical analysis used was the one-way analysis of variance ANOVA Obi (1986).

Results and Discussion

The data obtained are summarized on Table 1. Table 1 shows the changes in the serum activities of some liver enzymes (AST, ALT and ALP) and total bilirubin levels in rats given varied doses (0.0, 6.0, 12.0 and 30.0gkg^-1) of crude petroleum. When compared with control (0.0g crude petroleum/ kg) values, AST, ALT, ALP and total bilirubin levels in serum of crude petroleum treated rats increased (p<0.05) in a manner that depends on both the dose administered and duration of exposure. The group given the highest dose (30gkg^-1) and exposed for longer periods (2months) had the highest increases (Table 1).

Elevation of AST and ALT along with the increase in ALP activity may reflect some inflammatory diseases or injury to the liver. In this study the maximum activity of ALP obtained was very high. Thus, suggesting the possibility of hepatocellular damage. Some investigators have illustrated that enzyme patterns in the serum, reflect the physiological state of the organ, for instance increase in serum levels of AST, ALT and ALP was observed in serum of fish exposed to 2, 3, 4 triaminoazo benzene resulting in hepatocellular damage (Krishan and Veena, 1980). Other studies indicate an increase in the activities of the hepatic enzymes following liver damage in fish and albino mouse exposed to toxic substances (Dheeret al., 1987, Mohssen, 1997, Sharpet al., 1996).The result of this study is in conformity with these findings. Evidence from the experimental data indicate that crude petroleum could induce liver dysfunction, and when exposure becomes high and chronic, it could damage the liver. Thus, any source of crude petroleum into the body should be avoided.

Acknowledgements

的authors acknowledge with thank the assistance of Mr. A. T. Agbaje in procuring some of the reagents used in this study from London. Also the staff in Chemical Pathology Laboratory, University of Port Harcourt Teaching Hospital and Abia State University Teaching Hospital, Aba, Abia State, Nigeria, are highly appreciated for their various contributions.

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