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Introduction

Plant productivity is known to be severely compromised by soil salinity¹．Around 190 million hectares of irrigated land have already been lost to salt and are no longer suitable for farming, according to a report by the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services²．India faces a similar issue with soil salinity affecting major agricultural states like Gujarat (2.23 M ha), Uttar Pradesh (1.37 M ha), Maharashtra (0.16 M ha), West Bengal (0.44 M ha) and Rajasthan (0.38 M ha).Collectively, these states account for 75% of the saline soil of the 6.73 million hectares of affected land in India³．

Oryza sativaL.a widely grown tropical cereals is increasingly being threatened by rising levels of salt. Rise in sea levels and frequent flooding has led to salt intrusion into fresh inland water. Additionally, the use of salted water for irrigation in rice adversely affects plant growth and subsequent crop yield⁴．Pusa Basmati (PB) an indigenously developed variety of rice through breeding is one of the most preferred rice varieties. It is coveted for its grain attributes and aroma. The PB cultivars 1121 and 1718 have been developed for improved resistance against several bacterial diseases⁵．However, their ability to sustain salinity stress is not known. In rice seed germination is severely affected by salinity impairing seedling establishment and growth⁶．Breeding of salt tolerant cultivars and the development of technologies that enable efficient seed germination is the key to ensuring high yield^{7, 8}．

种子priming technology offers the possibility of enhancing seed germination and crop productivity⁹．It is one of the most promising pre-sowing seed treatments to boost crop output under stressful conditions and is one of the efficient procedures for micronutrient administration during planting in field crops^10,11．种子priming facilitates quicker germination in plants under abiotic stress by induction of protective cellular and metabolic events thus facilitating speedy emergence and uniform stand establishment of seedlings which eventually enhances the crop productivity¹²．Various priming treatments like hydropriming, PGR-based (plant growth regulators) hormonal priming, osmopriming, organic priming and chemical priming are used to increase the resilience of crop plants towards various abiotic stresses^13-16．种子启动方法减轻的效果abiotic stress has been well documented on various plants likeZea mays, Vigna radiata, Triticum sp,andOryza sativa^17-19．In this study, selected organic, chemical and hormonal primers have been tested for their efficacy in promoting seed germination and growth inOryza sativa天印度香米品种1121 PB和PB 1718年代alt stress.

Materials and Methods

Procurement of plant material

种子s ofOryza sativavar. Pusa Basmati (cultivars PB 1121 and PB 1718) were procured from Seed Bank, IARI, New Delhi, India. The seeds were washed with a mild detergent, rinsed thoroughly with water and treated with various priming agents for 24 hours at room temperature. Seed priming treatments were given in a randomized manner with three replicates per treatment. The primers used in the study were:

Organic Priming- Cow urine (2%), Moringa leaf extract (MLE; 100%), Neem leaf extract (NLE; 50%)

Chemical Priming- NaCl (100 mM), KNO₃(1 %), MgSO₄(10 mM)

Hormones- Salicylic Acid (1 mM),

Amino acid- Proline (50 mM)

The concentrations of seed priming agents used in this study were based on the published reports.The seeds were washed thoroughly in water, air dried for 24 hrs and stored till further use. The primed seeds were subjected to salt stress by exposure to 150 mM NaCl for 24 hrs. All the experiments were performed in triplicates.

Estimation of germination parameter

The seed germination parameters were assessed using the following formulas:

Germination percentage (GP) =(N₁/N₂) × 100

N₁= number of germinated seeds

N₂= total number of seeds

平均每日发芽(MDG) =最终GP /数量days to reach final GP

Peak value (PV) = Final GP/ number of days required to reach the peak value of the germination

Germination value = PV × MDG

Germination energy percentage = (Number of seeds germinated at 2 days after sowing / total number of the seeds tested) × 100

Estimation of growth parameter

Using a thread and a scale the shoot length was measured from the tip of the growing shoot to the base of the cotyledons. Similarly, for root length the length from tip of the growing root to the base of cotyledons was measured.

Biochemical analysis

For biochemical analysistotal protein content was assessed spectrophotometrically by using Lowry’s method²⁰, total phenolics content using Folin-Ciocalteu method^21,22and antioxidant levels using 1, Diphenyl 2- Picryl Hydrazyl (DPPH) assay²³．

Statistical Analysis

Control and primed samples were analyzed for significant differences between various groups by two tailed t-test and ANOVA using MS Excel software.

Results

Effect on seed germination indices

The two rice cultivars varied in their responses.In case of cultivarPB 1121,no significant change was seen in any of the germination indices, under salt stress as compared to control (Fig 1 A). The rice cultivar PB 1718 responded positively and an increase was observed in various germination indices (Fig 1 B). An increase in percentage germination and mean daily germination was observed in the seeds primed with NaCl (100mM), KNO₃(1%), salicylic acid (1mM), Moringa leaf extract (100%) and Neem leaf extract (50%) (Fig 1B). Increase in peak value and germination energy percentage was seen in primed seeds as compared to control (Fig 1B). Nearly 20% increase in germination energy percentage was observed in seeds primed with salicylic acid (90%), KNO₃(90%) and Neem leaf extract (90%) as compared to control (70%) (Fig 1B).

Figure 1: Effect of seed priming on various germination indices in rice cultivars A. PB 1121 B. PB 1718. Click here to view Figure

Effect on growth parameters

In comparison to control, no significant increase in shoot length was observed in seeds subjected to various priming treatments in both rice cultivars (Fig 2, 3).

Figure 2:Effect of different priming agents onOryza sativa(A) PB 1718 (B) PB 1121seedlings under 150mM NaCl stress. (Red block shows the control) Click here to view Figure

In cultivar PB 1121, an increase in root length was seen in seedlings treated with Salicylic acid (1mM), KNO₃(1%), Moringa leaf extract (100%) and Proline(50mM)(Fig. 3A). However, a significant increase in root length was observed in cultivar PB 1718 seedlings treated with NaCl, Salicylic acid (1mM), KNO₃(1%), Moringa leaf extract (100%) and Neem leaf extract (100%) (Fig. 3B). The increased root growth is important for enhancing water uptake and mineral acquisition.

Figure 3: Effect of seed priming on shoot and root length in A. PB 1121, B. PB 1718 Click here to view Figure

In cultivar PB 1121, no significant increase was observed in fresh and dry weight in primed seeds(Fig. 4 A). In cultivar PB 1718 a moderate increase in dry weight was observed in seeds primed with MgSO₄(10mM), Cow urine (2%), Neem leaf extract (50%) and Moringa leaf extract (100%) (Fig. 4 B).

Figure 4: Effect of priming on fresh and dry weight of seedlings in rice cultivars A. PB 1121 B. PB 1718. Click here to view Figure

Biochemical analysis

In cultivar PB 1121, an increase in total protein content was observed in seeds primed with Salicylic acid, Moringa leaf extract and Neem leaf extract (40% increase) (Fig. 5B). In cultivar PB 1718 slight increase in total protein was notedin Cow urine (8% increase) and NaCl (2% increase) as compared to control (Fig 5B).

Figure. 5: Effect of priming treatments on total protein content (ug BSA equivalent/gm fresh wt.) in control and primed seeds of rice cultivars PB 1121 and PB 1718 Click here to view Figure

A significant increase of more than 30% was observedin total phenolics content (TPC)in cultivar PB 1121 in NaCl, KNO₃, Salicylic acid, MgSO₄and Neem leaf extractpriming treatments as compared to control (Fig 6). In cultivar PB 1718, 40 % increase in TPC was noted in KNO₃and 19 % in MgSO₄primed seeds as compared to control (Fig 6).

Figure 6: Effect of seed priming on total phenolics content (ug GAE equiv./gm fresh wt.) in rice cultivars PB 1121 and PB 1718. Click here to view Figure

Analysis of antioxidant activity was done by DPPH assay. In cultivar PB 1121, three priming treatments were found to have a moderately higher % reduction than control. The highest % reduction was observed in seeds primed with proline [58.11 ± 4.54 %] followed by, salicylic acid [45.27 ± 18.36 %] and KNO₃[39.88 ± 9.60 %] as compared to control [38.29± 6.93%] (Fig 7).In cultivar PB 1718, all the priming treatments were observed to have significantly higher % reduction than control. The highest % reduction was observed in seeds primed with MgSO₄[77.82 ± 3.91 %], followed by NaCl [77.20 ± 4.59 %], Moringa leaf extract [74.77 ± 3.90 %], Cow urine [74.21 ± 5.37 %], KNO₃[74.38 ± 5.52 %] as compared to control [22.38 ± 2.51 %](Fig. 7).

Figure. 7: Effect of priming on DPPH activity (% reduction) in rice cultivars PB 1121 and PB 1718 Click here to view Figure

Discussion

In the present study, efficacy of different seed priming treatments was tested for amelioration of salt stress in two popular varieties ofOryza sativa.Out of the two cultivars that were tested, cultivar PB 1718showed better plant growth and improved germination when subjected to priming treatment with Salicylic acid (1mM), KNO₃(1%), Moringa leaf extract (100%) and Neem leaf extract (50%) under salt stress of NaCl (150 mM).In cultivar PB 1121, no significant change was seen in germination indices under salt stress as compared to the control.However,this cultivar showed better root growth upon priming treatments with Salicylic acid (1mM), KNO₃(1%), Proline (50mM), Moringa leaf extract (100%), MgSO₄(10mM) and Neem leaf extract (50%).

KNO₃and Salicylic acid are reported to have a significant impact on emergence, seedling growth, and biochemical attributes as well as antioxidant properties of rice seedlings.KNO₃acts by maintaining ion homeostasis which is found to be essential for osmotic adjustment under salt stress. It facilitates membrane stability under stress, prevents chlorophyll degradation and regulates various physiological processes²⁴．The application of salicylic acid accelerates the growth of cells by both division and elongation. It also helps in scavenging enzymessuch as amylase, dehydrogenase and acid phosphatase in primed seedsby reactive oxygen species (ROS) and thereby stimulating seedling growth²⁵．The Salicylic Acid pre-treatment also leads to the accumulation of various osmolytes, antioxidants and antioxidative enzymes to normal levels²⁶．

Moringa and Neem leaves have been reported to facilitate cell division due to the presence of zeatin which is a natural cytokinin, in addition to minerals like potassium, calcium, magnesium, vitamin c etc²⁷．Similar results involving seed priming with leaf extracts have been found to improve seed quality and overall yield in maize hybrids²⁸．Priming treatments with MLE has been reported to improve photosynthetic pigment levels thereby promoting plant growth under stress conditions.

Conclusion

In the present study seed priming treatment of two popular rice cultivars (PB 1121 and PB 1718) showed promising results for overcoming salt stress with improved germination, seedling establishment and growth. The cultivars assessed showed variable responses towards different priming treatments. Out of the two cultivars assessed, PB 1718 exhibited robust seedling establishment upon priming treatments as compared to PB 1121. In cultivar PB 1718, priming treatments with KNO₃(1%), SA (1 mM), NLE (50%) and MLE (100%) resulted in better germination, growth and biochemical parameters, leading to improved alleviation of salinity stress. In cultivar PB 1121,Salicylic acid (1mM), KNO₃(1%), MLE (100%), MgSO₄(10mM), Proline (50Mm) and NLE (50%) treatments resulted in improved root growth. Therefore, the present study has the potential for developing cost-effective, easy-to-use, accessible and sustainable priming methods for overcoming salt stress in rice.

Acknowledgement

The authors gratefully acknowledge the financial support provided by Center for Research, Maitreyi College, University of Delhi.

Conflict of Interest

The authors declare no conflict of interest.

Funding Soureces

The project received financial support from the Center for Research, Maitreyi College, University of Delhi. under Summer Internship Program 2022.

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