Biodegradation of Chlorpyrifos by Pseudomonas Resinovarans Strain AST2.2 Isolated from Enriched Cultures.
Anish Sharma1*, Jyotsana Pandit1, Ruchika Sharma1and Poonam Shirkot1
http://dx.doi．org/10.12944/CWE.11.1.33
A bacterial strain AST2.2 with chlorpyrifos degrading ability was isolated by enrichment technique from apple orchard soil with previous history of chlorpyrifos use. Based on the morphological, biochemical tests and 16S rRNA sequence analysis, AST2.2 strain was identified asPseudomonas resinovarans．应变AST2.2利用毒死蜱的年代ole source of carbon and energy. This strain exhibited growth upto 400mg/l concentration of chlorpyrifos and exhibited high extracellular organophosphorus hydrolase (OPH) activity. Gas chromatography-flame ionization detector (GC-FID) studies revealed thatPseudomonas resinovaransAST2.2 degraded 43.90 % of chlorpyrifos (400 mg/l) within 96 hrs. Intermediates of chlorpyrifos degradation were identified using GC-MS. This strain have potential to degrade chlorpyrifos and thus can be used for bioremediation and ecological restoration of sites contaminated with chlorpyrifos.
Chlorpyrifos; OPH; Gas Chromatography; Pseudomonas Resinovarans
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Sharma A, Pandit J, Sharma R, Shirkot P. Biodegradation of Chlorpyrifos by Pseudomonas Resinovarans Strain AST2.2 Isolated from Enriched Cultures. Curr World Environ 2016;11(1) DOI:http://dx.doi．org/10.12944/CWE.11.1.33
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Sharma A, Pandit J, Sharma R, Shirkot P. Biodegradation of Chlorpyrifos by Pseudomonas Resinovarans Strain AST2.2 Isolated from Enriched Cultures. Curr World Environ 2016;11(1). Available from://www.a-i-l-s-a.com/?p=13906
