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The Presence of Mercury Resistant Bacteria in Sediment of Gold Processing Plant at Waekerta Village of Buru District, Maluku Province and their Activity in Reducing Mercury

Sarmawaty Kotala1*, Retno Kawuri2and Ida Bagus Wayan Gunam3

DOI:http://dx.doi.org/10.12944/CWE.9.2.07

Mercury was one of the heavy metal polute in environment and had the toxic characteristic to the living creatures. Golden mining in Waeapo subdistrict used mercury to extract the gold and exile the waste to the environment freely. Several precedented research showed that waste sediment of gold processing contains mercury resistance bacteria. Mercury resistance bacteria can be used as bioremediation agent because those bacteria can reduce mercury. Mercury resistance bacteria hasmer operonwhich contained in plasmid. The goal of this research is to isolate mercury resistance bacteria which is able to grow on medium nutrient agar (NA) containing 500 ppm of HgCl2and to analyze the capability in HgCl2reduction in nutrient broth (NB) medium. Bacteria isolation was done by platting method on Nutrient Agar containing 10 ppm of HgCl2. Bacteria identification was done by kit Microgen TM GnA + B-ID System and to know bacteria capability in reducing mercury was done by CV-AAS (Cold Vapour Atomic Absorption Spectrophotometer). Result showed, that the bacteria found in this research wereBacillussp andAeromonas hydrophila. Both of these bacteria were able to reduce HgCl2in the amount of 98,7% forBacillussp and 98,33% forAeromonas hydrophila. In the future those bacteria can be use as bioremediation agent.


Mercury Resistant Bacteria; Bacillus sp; Aeromonas hydrophila; Gold Processing

Copy the following to cite this article:

Kotala S, Kawuri R, Gunam I. B. W. The Presence of Mercury Resistant Bacteria in Sediment of Gold Processing Plant at Waekerta Village of Buru District, Maluku Province and their Activity in Reducing Mercury. Curr World Environ 2014;9 (2) DOI:http://dx.doi.org/10.12944/CWE.9.2.07

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Kotala S, Kawuri R, Gunam I. B. W. The Presence of Mercury Resistant Bacteria in Sediment of Gold Processing Plant at Waekerta Village of Buru District, Maluku Province and their Activity in Reducing Mercury. Curr World Environ 2014;9(2). Available from://www.a-i-l-s-a.com?p=502/